Characterization of Peptacetobacter hominis gen. nov., sp. nov., isolated from human faeces, and proposal for the reclassification of Clostridium hiranonis within the genus Peptacetobacter

作者：Xiaojiao Chen, Zhanqiang Wang, Zuyi Zhou, Nianyi Zeng, Qing-Fa Huang, Zhongwei Wang, Wenli Tang, Hongwei Zhou · 发表于：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 年份：2020 · DOI：10.1099/ijsem.0.003925 · 被引用次数：13 · 研究领域：Microbial Metabolism and Applications、Genomics and Phylogenetic Studies、Microbial Natural Products and Biosynthesis

A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191 T , was isolated from human faeces and characterized by using a polyphasic taxonomic approach. Growth occurred at 25–45 °C (optimum, 37–42 °C), at pH 5.5–10.0 (optimum, pH 6.5–7.0) and with 0–2 % (w/v) NaCl (optimum, 0 %). The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid. The dominant cellular fatty acids (>10 %) of strain ZHW00191 T were C 16 : 0 , C 18 : 1 ω 9 с and C 18 : 2 ω6,9 с . Its polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and ten unidentified glycolipids. Respiratory quinones were not detected. The cell-wall peptidoglycan contained meso -2,6-diaminopimelic acid, and the whole-cell sugars were ribose and glucose. The genomic DNA G+C content was 32.8 mol%. Analysis of the 16S rRNA gene sequence indicated that ZHW00191 T was most closely related to Clostridium hiranonis TO-931 T (95.3 % similarity). Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95–96% and 70 % for species delineation, respectively. Based on phenotypic, chemotaxonomic and genetic studies, a novel genus, Peptacetobacter gen. nov., is proposed. The novel isolate ZHW00191 T (=JC...