Astaxanthin Is Ketolated from Zeaxanthin Independent of Fatty Acid Synthesis in Chromochloris zofingiensis

作者：Yu Zhang, Ying Ye, Wei Ding, Xuemei Mao, Yantao Li, Henri Gerken, Jin Liu · 发表于：PLANT PHYSIOLOGY · 年份：2020 · DOI：10.1104/pp.20.00325 · 被引用次数：103 · 研究领域：Algal biology and biofuel production、Antioxidant Activity and Oxidative Stress、Photosynthetic Processes and Mechanisms

The biosynthesis of astaxanthin, a high-value keto-carotenoid with broad industrial applications, remains unambiguous in algae. Here, we dissected the astaxanthin biosynthetic pathway and the coordination between astaxanthin and triacylglycerol (TAG) biosynthesis in the emerging model alga Chromochloris zofingiensis. In vivo and in vitro experiments demonstrated that astaxanthin, utilizing the methylerythritol phosphate pathway-derived isopentenyl diphosphate as the building block, was synthesized from β-carotenoid ketolase-mediated ketolation of zeaxanthin rather than β-carotenoid hydroxylase-mediated hydroxylation of canthaxanthin, thus leading to the buildup of astaxanthin and canthaxanthin as end products in C. zofingiensis. The synthesized astaxanthin, stored in TAG-filled lipid droplets, was esterified mainly with the fatty acid C18:1, which was not catalyzed by any acyltransferase previously proposed. Astaxanthin accumulated in a well-coordinated manner with TAG, supported by the coordinated up-regulation of both biosynthetic pathways at the transcriptional level. Nevertheless, astaxanthin and TAG showed no interdependence: inhibition of de novo fatty acid biosynthesis severely attenuated TAG biosynthesis but promoted the accumulation of astaxanthin, particularly in the diester form, leading to a fivefold increase in the astaxanthin/TAG ratio; however, inhibition of astaxanthin biosynthesis showed little effect on TAG accumulation. Our data suggest that an increase in ...