Resistance of Klebsiella pneumoniae Strains Carrying blaNDM–1 Gene and the Genetic Environment of blaNDM–1

作者：Tianxin Xiang, Chuanhui Chen, Jiangxiong Wen, Yang Liu, Qí Zhāng, Na Cheng, Xiaoping Wu, Wei Zhang · 发表于：Frontiers in Microbiology · 年份：2020 · DOI：10.3389/fmicb.2020.00700 · 被引用次数：29 · 研究领域：Antibiotic Resistance in Bacteria、Tuberculosis Research and Epidemiology、Bacterial biofilms and quorum sensing

Objective Regional dissemination is the major cause of the widespread prevalence of a plasmid-encoding NDM-1 enzyme. We here investigated the drug resistance, joint efficiency, and gene environment of a Klebsiella pneumoniae strain carrying blaNDM-1. Materials and Methods Carbapenem-insensitive strains were analyzed using the VITEK 2 Compact. Strains carrying blaNDM-1 were identified by polymerase chain reaction and sequencing. Antimicrobial susceptibility testing and plasmid conjugation experiment was then conducted. Strains carrying blaNDM-1 were subjected to Southern blot analysis. After gene mapping of blaNDM-1, library construction and sequencing, plasmids were subsequently spliced and genotyped using the Glimmer 3.0 software and then analyzed by Mauve software. Results Among 1735 carbapenem-insensitive strains, 54 strains of blaNDM-1-positive bacteria were identified, which consist of 44 strains of K. pneumoniae, 8 strains of Acinetobacter baumannii and 2 strains of Escherichia coli. Strains carrying blaNDM-1 had a resistance rate of more than 50% in most antibiotics. Plasmid conjugation between strains carrying blaNDM-1 and E. coli strain J53 had a success rate of 50%. Southern blot analysis indicated that every strain had multiple plasmids containing blaNDM-1. Among the 5 plasmids containing blaNDM-1in K. pneumonia for sequencing, 2 plasmids with complete sequences were obtained. And we found: (i) The p11106 and p12 plasmids were highly similar to pNDM-BTR; (ii) The p...