Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice

作者：Yi Chen, Qin Li, Zhiping Kuang, Xin Zhao, Ruokun Yi, Xiaowei He, Guangjing Feng · 发表于：Journal of Food Quality · 年份：2020 · DOI：10.1155/2020/1206247 · 被引用次数：13 · 研究领域：Chromatography in Natural Products、Ginseng Biological Effects and Applications、Phytochemical Studies and Bioactivities

The aim of this study was to investigate the antioxidant effects of flavonoid extract of lotus leaf (FELL) in vitro and in mice with gastric injury and elucidate the inhibitory effect of FELL on alcohol-induced gastric injury by antioxidant capacity. Gastric juice volume and acid value were measured in the established alcohol-induced acute gastric injury model in mice. Hematoxylin and eosin staining demonstrated the morphological changes in the gastric mucosa. FELL improved the alcohol-induced gastric lesions. Serological testing results showed that FELL significantly increased the levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and nitric oxide (NO) and significantly reduced the levels of MDA and MPO in mice with gastric injury. Besides, FELL significantly reduced the levels of interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor alpha (TNF- α ), and interferon gamma (IFN- γ ). The quantitative polymerase chain reaction (qPCR) and Western blot results proved that FELL upregulated the mRNA and protein expressions of SOD1, SOD2, CAT, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), neuronal nitric oxide synthase (nNOS), and endothelial nitric oxide synthase (eNOS) and downregulated the expression of inducible nitric oxide synthase (iNOS) in the gastric tissue of mice with gastric injury. Five compounds, including kaempferitrin, hyperoside, astragalin, phloridzin, and querc...