High-efficiency dynamic sensing of biothiols in cancer cells with a fluorescent β-cyclodextrin supramolecular assembly

作者：Zhixue Liu, Wei‐Lei Zhou, Jingjing Li, Haoyang Zhang, Xianyin Dai, Yaohua Liu, Yu Liu, Yu Liu, Yu Liu · 发表于：Chemical Science · 年份：2020 · DOI：10.1039/d0sc00414f · 被引用次数：46 · 研究领域：Sulfur Compounds in Biology、Molecular Sensors and Ion Detection、Inorganic Fluorides and Related Compounds

host-guest inclusion complexation. Importantly, the coumarin-modified β-cyclodextrin not only showed higher sensitivity than the parent coumarin derivatives owing to the presence of numerous hydroxyl groups on the cyclodextrin but also provided a hydrophobic cavity for encapsulation of a cancer-targeting agent. The assembly showed a reversible and fast response to biothiols with a micromolar dissociation constant, as well as outstanding cancer cell permeability, which can be used for high-efficiency real-time monitoring of biothiols in cancer cells. This supramolecular assembly strategy endows the fluorescent probe with superior performance for dynamic sensing of biothiols.