Single-cell transcriptomes of the human skin reveal age-related loss of fibroblast priming

作者：Llorenç Solé‐Boldo, Günter Raddatz, Sabrina Schütz, Jan‐Philipp Mallm, Karsten Rippe, Anke S. Lonsdorf, Manuel Rodríguez‐Paredes, Frank Lyko · 发表于：Communications Biology · 年份：2020 · DOI：10.1038/s42003-020-0922-4 · 被引用次数：572 · 研究领域：Skin Protection and Aging、Single-cell and spatial transcriptomics、melanin and skin pigmentation

Fibroblasts are an essential cell population for human skin architecture and function. While fibroblast heterogeneity is well established, this phenomenon has not been analyzed systematically yet. We have used single-cell RNA sequencing to analyze the transcriptomes of more than 5,000 fibroblasts from a sun-protected area in healthy human donors. Our results define four main subpopulations that can be spatially localized and show differential secretory, mesenchymal and pro-inflammatory functional annotations. Importantly, we found that this fibroblast 'priming' becomes reduced with age. We also show that aging causes a substantial reduction in the predicted interactions between dermal fibroblasts and other skin cells, including undifferentiated keratinocytes at the dermal-epidermal junction. Our work thus provides evidence for a functional specialization of human dermal fibroblasts and identifies the partial loss of cellular identity as an important age-related change in the human dermis. These findings have important implications for understanding human skin aging and its associated phenotypes.