Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

作者：Liyuan Wang, Yilin Zou, Han Yeong Kaw, Gang Wang, Huaze Sun, Long Cai, Chengyu Li, Long-Yue Meng, Donghao Li · 发表于：Plant Methods · 年份：2020 · DOI：10.1186/s13007-020-00595-4 · 被引用次数：65 · 研究领域：Plant and animal studies、Plant Reproductive Biology、Pesticide Residue Analysis and Safety

Plant hormones are naturally occurring small molecule compounds which are present at trace amounts in plant. They play a pivotal role in the regulation of plant growth. The biological activity of plant hormones depends on their concentrations in the plant, thus, accurate determination of plant hormone is paramount. However, the complex plant matrix, wide polarity range and low concentration of plant hormones are the main hindrances to effective analyses of plant hormone even when state-of-the-art analytical techniques are employed. These factors substantially influence the accuracy of analytical results. So far, significant progress has been realized in the analysis of plant hormones, particularly in sample pretreatment techniques and mass spectrometric methods. This review describes the classic extraction and modern microextraction techniques used to analyze plant hormone. Advancements in solid phase microextraction (SPME) methods have been driven by the ever-increasing requirement for dynamic and in vivo identification of the spatial distribution of plant hormones in real-life plant samples, which would contribute greatly to the burgeoning field of plant hormone investigation. In this review, we describe advances in various aspects of mass spectrometry methods. Many fragmentation patterns are analyzed to provide the theoretical basis for the establishment of a mass spectral database for the analysis of plant hormones. We hope to provide a technical guide for further discove...