Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

作者：Ishita Matai, Satish Kumar Pandey, Deepa Garg, Komal Rani, Abhay Sachdev · 发表于：Nano Express · 年份：2020 · DOI：10.1088/2632-959x/ab8bea · 被引用次数：24 · 研究领域：Nanoparticles: synthesis and applications、Selenium in Biological Systems、Phytochemistry and Bioactivity Studies

Abstract The exploitation of plant extracts for the synthesis of nano selenium having antibacterial and antioxidant activities is an exciting approach to counteract the prevalence of infections caused by antibiotic-resistant bacteria, which holds relevance for medical and food industries. In the present work, a green and facile method for the preparation of nano selenium (nSe) using the fruit extract of Indian gooseberry ( Phyllanthus Emblica ) has been reported. The optical and structural properties of the as-synthesized nSe were studied through various characterization techniques. Eventually, the antioxidant potential of nSe was investigated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl free radical scavenging assays. Parallely, the antibacterial activity of nSe against Escherichia coli , Staphylococcus aureus and Pseudomonas aeruginosa was evaluated. The antioxidant assays indicated that even low dosage of nSe showed excellent activity with EC 50 values of 0.21 μ g ml −1 and 3.34 μ g ml −1 , respectively. Moreover, nSe exhibited significant inhibition in bacterial growth at low minimum inhibitory concentration (MIC) values against Escherichia coli (16 μ g ml −1 ), Staphylococcus aureus (32 μ g ml −1 ) and Pseudomonas aeruginosa (48 μ g ml −1 ) compared to MIC values for standard drug ampicillin. Importantly, nSe did not induce any cytotoxic effects on normal human keratinocytes (HaCaT) at the tested concentrations; representing their biocompatible nature. The data ...