Dynamics of Intestinal Inflammatory Cytokines and Tight Junction Proteins of Turbot (Scophthalmus maximus L.) During the Development and Recovery of Enteritis Induced by Dietary β-Conglycinin

作者：Jing Zheng, Pei Yang, Jihong Dai, Guijuan Yu, Weihao Ou, Weiqi Xu, Kangsen Mai, Yanjiao Zhang · 发表于：Frontiers in Marine Science · 年份：2020 · DOI：10.3389/fmars.2020.00198 · 被引用次数：19 · 研究领域：Aquaculture Nutrition and Growth、Aquaculture disease management and microbiota、Animal Nutrition and Physiology

This study was conducted to investigate the dynamics characterization of enteritis of juvenile turbot (Scophthalmus maximus L.), during the development of enteritis induced by dietary β-conglycinin and the recovery by fish meal. Two isonitrogenous and isolipidic experimental diets were formulated, fish meal (FM) diet and fish meal supplemented with 8% β-conglycinin (7S) diet. The feeding trial consist of three groups, (1) turbot fed FM and 7S diet for ten weeks respectively, (2) turbot fed 7S diet for three weeks and then fed FM diet for another seven weeks (7S&FM). The distal intestines and serum of turbot in 7S&FM group were sampled at the 0 day, 3rd day, 1st week, 2nd week, 3rd week, 6th week and 10th week. After ten weeks feeding, the growth performance and feed utilization of turbot in FM and 7S&FM group were significantly higher than that of turbot in 7S group. According to the observation of intestine histology, the typical symptoms of enteritis were observed in the third week and then mitigated by fish meal gradually until the tenth week. The activities of DAO and content of D-lactate in serum showed the similar trend in ten weeks. The gene expression of pro-inflammatory cytokines IL-1β, IL-8, IL-22 and IFN-γ was significantly increased by 7S in the first three weeks and then significantly decreased by FM since the sixth week, as well as the expression of NF-κB. The opposite trend of gene expression was observed in anti-inflammatory cytokine TGF-β. The gene expression...