Effect of α2‑macroglobulin in the early stage of jaw osteoradionecrosis

作者：Jie Li, Ping Yin, Xueying Chen, Xiangbo Kong, Wanzhen Zhong, Yaping Ge, Yangyang She, Xuehong Xian, Lei Qi, Zhi Xiu Lin, Justine Moe, Silian Fang · 发表于：International Journal of Oncology · 年份：2020 · DOI：10.3892/ijo.2020.5051 · 被引用次数：15 · 研究领域：Oral health in cancer treatment、Salivary Gland Disorders and Functions、Cancer Diagnosis and Treatment

Advanced osteoradionecrosis (ORN) is one of the most serious complications in patients with head and neck cancer, resulting in poor prognosis. Numerous studies have therefore focused on the pathogenesis and interventions of ORN early stage. The present study aimed to investigate whether α2‑macroglobulin (α2M) could prevent early‑stage jaw osteoradionecrosis caused by radiotherapy (RT). Following local injection of α2M, a single dose of 30 Gy was delivered to rats for pathological exploration. For 28 days, the irradiated mandible and soft tissues were examined for potential changes. Furthermore, primary human bone marrow mesenchymal stem cells pretreated with α2M followed by 8 Gy irradiation (IR) were also used. Tartrate‑resistant acid phosphatase assay, terminal uridine deoxynucleotidyl nick end labeling assay and immunohistochemical staining were performed on irradiated mandibular bone, tongue or buccal mucosa tissues from rats. Cell proliferation was assessed by evaluating the cell morphology by microscopy and by using the cell counting kit‑8. Fluorescence staining, flow cytometry and western blotting were conducted to detect the reactive oxygen species level, cell apoptosis and protein expression of superoxide dismutase 2 (SOD2), heme oxygenase‑1 (HO‑1) and phosphorylated Akt following irradiation. The results demonstrated that α2M attenuated physical inflammation, osteoclasts number and fat vacuole accumulation in mandibular bone marrow and bone marrow cell apoptosis foll...