Genomic characterization of 16S rRNA methyltransferase-producing Escherichia coli isolates from the Parisian area, France

作者：François Caméléna, F. Morel, Manel Mérimèche, Jean‐Winoc Decousser, Hervé Jacquier, Olivier Clermont, Mélanie Mercier Darty, Mary Mainardis, Emmanuelle Cambau, Olivier Tenaillon, Érick Denamur, Béatrice Berçot, the IAME Resistance Group, Clotilde Rousseau, Thibaut Poncin, Aymeric Braille, Marlène Amara, Hedi Mammeri, Laurence Armand-Lefèvre, Sylvain Kumanski, Guilhem Royer, Suzanne Bialek, Luce Landraud, Catherine Branger, Étienne Carbonnelle, Stéphane Bonacorsi · 发表于：Journal of Antimicrobial Chemotherapy · 年份：2020 · DOI：10.1093/jac/dkaa105 · 被引用次数：25 · 研究领域：Antibiotic Resistance in Bacteria、Urinary Tract Infections Management、Escherichia coli research studies

BACKGROUND: The resistance to all aminoglycosides (AGs) conferred by 16S rRNA methyltransferase enzymes (16S-RMTases) is a major public health concern. OBJECTIVES: To characterize the resistance genotype, its genetic environment and plasmid support, and the phylogenetic relatedness of 16S-RMTase-producing Escherichia coli from France. METHODS: We screened 137 E. coli isolates resistant to all clinically relevant AGs from nine Parisian hospitals for 16S-RMTases. WGS was performed on clinical isolates with high-level AG resistance (MIC ≥256 mg/L) and their transformants. RESULTS: Thirty of the 137 AG-resistant E. coli produced 16S-RMTases: 11 ArmA, 18 RmtB and 1 RmtC. The 16S-RMTase producers were also resistant to third-generation cephalosporins (90% due to a blaCTX-M gene), co-trimoxazole, fluoroquinolones and carbapenems (blaNDM and blaVIM genes) in 97%, 83%, 70% and 10% of cases, respectively. Phylogenomic diversity was high in ArmA producers, with 10 different STs, but a similar genetic environment, with the Tn1548 transposon carried by a plasmid closely related to pCTX-M-3 in 6/11 isolates. Conversely, RmtB producers belonged to 12 STs, the most frequent being ST405 and ST complex (STc) 10 (four and four isolates, respectively). The rmtB gene was carried by IncF plasmids in 10 isolates and was found in different genetic environments. The rmtC gene was carried by the pNDM-US plasmid. CONCLUSIONS: ArmA and RmtB are the predominant 16S-RMTases in France, but their spread fol...