Epitope-directed antibody selection by site-specific photocrosslinking

作者：Longxin Chen, Chaoyang Zhu, Hui Guo, Runting Li, Limeng Zhang, Zhenzhen Xing, Yue Song, Zihan Zhang, Fuping Wang, Xiaofeng Liu, Yuhan Zhang, Z. Runlin, Feng Wang · 发表于：Science Advances · 年份：2020 · DOI：10.1126/sciadv.aaz7825 · 被引用次数：34 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Glycosylation and Glycoproteins Research、Advanced Biosensing Techniques and Applications

-benzoyl-l-phenylalanine (pBpa), on the target antigen epitope. By one or two rounds of panning against antibody phage display libraries, those hits that covalently bind to the proximity site of pBpa on specific epitopes of target antigens after ultraviolet irradiation are enriched and selected. This method was applied to specific epitopes on human interleukin-1β and complement 5a. In both cases, more than one-third of hits identified bind to the target epitopes, demonstrating the feasibility and versatility of this method.