Simultaneous Discrimination of Hypochlorite and Single Oxygen during Sepsis by a Dual-Functional Fluorescent Probe

作者：Lingliang Long, Yuanyuan Han, Weiguo Liu, Chen Qian, Dan-Dan Yin, Lulu Li, Yuan Fang, Zhixiang Han, Aihua Gong, Kun Wang · 发表于：Analytical Chemistry · 年份：2020 · DOI：10.1021/acs.analchem.0c00492 · 被引用次数：59 · 研究领域：Molecular Sensors and Ion Detection、Analytical Chemistry and Sensors、Luminescence and Fluorescent Materials

Hypochlorite (ClO – ) and singlet oxygen ( 1 O 2 ) commonly coexist in living systems and exert important interplaying roles in many diseases. To dissect their complex inter-relationship, it is urgently required to construct a fluorescent probe that can discriminate ClO – and 1 O 2 in living organisms. Herein, by taking the 3-(aliphaticthio)-propan-1-one group as the unique recognition unit for both ClO – and 1 O 2, we proposed the first fluorescent probe, Hy-2, to simultaneously discriminate ClO – and 1 O 2 with high sensitivity and selectivity. Probe Hy-2 itself showed fluorescence in blue channel. After treatment with ClO – and 1 O 2, respectively, pronounced fluorescence enhancements were observed in the green channel and red channel correspondingly. Moreover, upon development of the probe with aggregation-induced emission (AIE) characteristics, the probe could work well in a solution with high water volume fraction. Probe Hy-2 was also able to accumulate into mitochondria and was utilized as an effective tool to image exogenous and endogenous ClO – and 1 O 2 in mitochondria. Significantly, as the first trial, probe Hy-2 was employed to simultaneously monitor the variation of ClO – and 1 O 2 level in cecal tissues of rat in the cecal ligation and puncture (CLP)-induced polymicrobial sepsis model. The results demonstrated that the expressed ClO – and 1 O 2 levels were tightly correlated with the severity of sepsis, inferring that the overproduction of ClO – and 1 O 2 is an...