Detecting kinsenoside from Anoectochilus roxburghii by HPLC‐ELSD with dual columns of NH 2 and AQ‐C 18

作者：Mi Wei, Xuemin Chen, Liwen Yi, Yuanyuan Yuan, Hua Zhang, Chunhua Fu, Longjiang Yu · 发表于：Phytochemical Analysis · 年份：2020 · DOI：10.1002/pca.2933 · 被引用次数：15 · 研究领域：Biological and pharmacological studies of plants、Traditional Chinese Medicine Analysis、Phytochemistry and Biological Activities

INTRODUCTION: Kinsenoside is a characteristic component of Anoectochilus roxburghii and accounts for this herb's medicinal and edible values. No international certified standard method is available for kinsenoside analysis as well as extraction and preservation. OBJECTIVE: To develop a more accurate analytical method of kinsenoside. The effects of extraction and drying methods of A. roxburghii on kinsenoside efficiency were investigated for the first time, as well as to examine the kinsenoside stability. MATERIAL AND METHODS: columns for detecting kinsenoside extract was systematically compared by high-performance liquid chromatography evaporative light-scattering detector (HPLC-ELSD) and HPLC-diode-array detector (DAD), respectively. Kinsenoside, its epimer goodyeroside A and the degradation product during preservation were identified through HPLC-electrospray ionization mass spectrometry (ESI-MS). RESULTS: column was multiplied by 1/(1 + Y) as the corrected result. Using this novel method, the average deviations were reduced by 7.64%. Moreover, the efficiency of kinsenoside extraction with water was almost twice that of extraction with ethanol. Freeze drying also led to a higher extraction efficiency (38.47% increase) than hot-air drying did. Furthermore, the degradation of kinsenoside extract exceeded 70% when stored at 37 °C for 3 months. CONCLUSION: This study provides a reliable experimental method and theoretical basis for the quality control of kinsenoside from A. rox...