Gold clusters prevent breast cancer bone metastasis by suppressing tumor-induced osteoclastogenesis

作者：Zhichao Zhang, Yawen Yao, Qing Yuan, Lu Cao, Xiangchun Zhang, Jinling Yuan, Kaixiao Hou, Chunyu Zhang, Zhongying Du, Xueyun Gao, Xiongsheng Chen · 发表于：Theranostics · 年份：2020 · DOI：10.7150/thno.42218 · 被引用次数：36 · 研究领域：Bone health and treatments、Bone Metabolism and Diseases、Trace Elements in Health

Rationale: Bone is the most frequent site for breast cancer metastasis, which accounts for the leading cause of death in advanced breast cancer patients. Serious skeletal-related events (SREs) caused by bone metastasis have a decisive impact on the life expectancy of breast cancer patients, making breast cancer almost incurable. Metastatic breast cancer cell induced pathological osteoclastogenesis is a key driver of bone metastasis and osteolytic bone lesions. We previously reported that gold clusters can prevent inflammation induced osteoclastogenesis and osteolysis in vivo. In this study, we investigated the effects of a BSA-coated gold cluster on metastatic breast cancer-induced osteoclastogenesis in vitro and tumor-induced osteolysis in vivo, and elucidated its possible mechanism. Methods: Breast cancer cell line MDA-MB-231 was used to evaluate the regulatory effects of gold clusters on breast cancer metastasis and tumor induced osteoclastogenesis in vitro. Cell counting kit-8, transwell, wound-healing and colony formation assays were performed to evaluate the effect of gold clusters on proliferation and metastasis of MDA-MB-231 cells. Tartrate-resistant acid phosphatase (TRAP) staining and filamentous-actin rings analysis were used to detect the regulatory effects of gold clusters on MDA-MB-231 cell-conditioned medium (MDA-MB-231 CM) triggered and receptor activator of nuclear factor-B ligand (RANKL)-induced osteoclastogenesis in mouse bone marrow-derived mononuclear cel...