Methylobacterium nonmethylotrophicum sp. nov., isolated from tungsten mine tailing

作者：Guang-Da Feng, Wendi Chen, Xianjiao Zhang, Jun Zhang, Shengnan Wang, Honghui Zhu · 发表于：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 年份：2020 · DOI：10.1099/ijsem.0.004112 · 被引用次数：16 · 研究领域：Metal Extraction and Bioleaching、Corrosion Behavior and Inhibition、Chromium effects and bioremediation

A novel pink-pigmented strain, designated 6HR-1 T , was isolated from tungsten mine tailings in Jiangxi Province, PR China. Cells were Gram-stain-negative, aerobic, non-spore-forming, rod-shaped and motile with a polar flagellum (monotrichous). It could not utilize methanol, methylamine, formaldehyde or formate as a sole carbon source. The methanol dehydrogenase mxaF gene was absent but the xoxF gene was present. Phylogenomic and 16S rRNA gene phylogenetic analyses clearly showed that strain 6HR-1 T was affiliated to the genus Methylobacterium and closely related to ‘ Methylobacterium terrae ’ 17Sr1-28 T (98.6 %), Methylobacterium platani JCM 14648 T (97.7 %), Methylobacterium variabile DSM 16961 T (97.7 %) and Methylobacterium currus KACC 19662 T (97.4 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain 6HR-1 T and its closely related type species were 87.4–88.7 and 33.2–36.3 %, respectively. It had summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ) as the major fatty acid and ubiquinone 10 as the predominant respiratory quinone. Polyphasic characterization supported that strain 6HR-1 T represents a novel species of the genus Methylobacterium , for which the name Methylobacterium nonmethylotrophicum sp. nov. is proposed with the type strain 6HR-1 T (=GDMCC 1.662 T =KCTC 42760 T ).