Production of riboflavin and related cofactors by biotechnological processes

作者：Shuang Liu, Wenya Hu, Zhiwen Wang, Tao Chen · 发表于：Microbial Cell Factories · 年份：2020 · DOI：10.1186/s12934-020-01302-7 · 被引用次数：135 · 研究领域：Amino Acid Enzymes and Metabolism、Microbial Metabolic Engineering and Bioproduction、Metabolism and Genetic Disorders

Riboflavin (RF) and its active forms, the cofactors flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), have been extensively used in the food, feed and pharmaceutical industries. Modern commercial production of riboflavin is based on microbial fermentation, but the established genetically engineered production strains are facing new challenges due to safety concerns in the food and feed additives industry. High yields of flavin mononucleotide and flavin adenine dinucleotide have been obtained using whole-cell biocatalysis processes. However, the necessity of adding expensive precursors results in high production costs. Consequently, developing microbial cell factories that are capable of efficiently producing flavin nucleotides at low cost is an increasingly attractive approach. The biotechnological processes for the production of RF and its cognate cofactors are reviewed in this article.