MiR‐20a inhibits the progression of human arthritis fibroblast‐like synoviocytes and inflammatory factor expression by targeting ADAM10

作者：Zikang Xie, Pengfei Shen, Yuxing Qu, Jianda Xu, Chong Zheng, Yi Gao, Bin Wang · 发表于：Environmental Toxicology · 年份：2020 · DOI：10.1002/tox.22923 · 被引用次数：16 · 研究领域：MicroRNA in disease regulation、RNA Interference and Gene Delivery、RNA Research and Splicing

MiR-20a has been reported as a key regulator to pro-inflammatory factor release in fibroblast-like synoviocytes (FLS), which caused rheumatoid arthritis (RA). However, the molecular mechanism of miR-20a in RA remains to be further elucidated. This study aimed to investigate the roles of miR-20a in RA pathology. RA (n = 24) and osteoarthritis (OA, n = 20) and normal healthy tissues (n = 16) were collected from operation. TargetScan and dual-luciferase reporter were performed to predict and confirm the potential binding sites of miR-20a on ADAM metallopeptidase domain 10 (ADAM10). Pearson's analysis was adopted to evaluate the correlation between miR-20a and ADAM10 expression. It was found that MiR-20a was downregulated in RA tissues, and overexpressed miR-20a inhibited cell viability, migration and invasion, and the expression of inflammatory factors in RA-FLS MH7A cells. ADAM10 was identified as the target gene of miR-20a, and upregulation of ADAM10 reversed the inhibitory effects of miR-20a. In conclusion, miR-20a inhibits the progression of RA-FLS as well as the inflammatory factor expression by targeting ADAM10.