MIR205HG facilitates carcinogenesis of lung squamous cell carcinoma <italic>in vitro</italic> revealed by long noncoding RNA profiling

作者：Yan Chang, Xinying Xue, Chunsun Li, Wei Zhao, Yongfu Ma, Fei Xu, Zhen Wu, Yu Dai, Yunjing Li, Yang Liu, Liangan Chen · 发表于：Acta Biochimica et Biophysica Sinica · 年份：2020 · DOI：10.1093/abbs/gmaa006 · 被引用次数：17 · 研究领域：Cancer-related molecular mechanisms research、RNA modifications and cancer、Mycobacterium research and diagnosis

As a subtype of non-small-cell lung cancer, lung squamous cell carcinoma (LUSC) accounts for one-fifth of all lung cancers. Unfortunately, no specific targetable aberration has yet been identified. Hence, it is of huge urgency and potential to identify aberrantly regulated genes in LUSC. Here, five pairs of LUSC samples and their corresponding adjacent tissues were subject to whole transcriptome sequencing. Our results showed that CTD-2562J17.6 and FENDRR were significantly downregulated while MIR205HG, LNC_000378, RP11-116G8.5, RP3-523K23.2, and RP5-968D22.1 were significantly upregulated in all five LUSC samples. Importantly, MIR205HG was upregulated in LUSC clinical samples as well as in LUSC cell lines. Interestingly, our results demonstrated that the expression level of MIR205HG is positively correlated with the malignancy. In addition, MIR205HG is required for LUSC cell growth and cell migration. Most importantly, our results showed that MIR205HG prohibits LUSC apoptosis via regulating Bcl-2 and Bax. Taken together, our data shed lights on the lncRNA regulatory nexus that controls the carcinogenesis of LUSC and provided potential novel diagnostic markers and therapeutic targets for LUSC.