In VivoThermodynamic Analysis of Glycolysis in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum Using13C and2H Tracers

作者：Tyler B. Jacobson, Travis C. Korosh, David M. Stevenson, Charles James Foster, Costas D. Maranas, Daniel G. Olson, Lee Rybeck Lynd, Daniel Amador‐Noguez · 发表于：mSystems · 年份：2020 · DOI：10.1128/msystems.00736-19 · 被引用次数：67 · 研究领域：Microbial Metabolic Engineering and Bioproduction、Protein Structure and Dynamics、Biofuel production and bioconversion

Thermodynamics constitutes a key determinant of flux and enzyme efficiency in metabolic networks. Here, we provide new insights into the divergent thermodynamics of the glycolytic pathways ofC. thermocellumandT. saccharolyticum, two industrially relevant thermophilic bacteria whose metabolism still is not well understood. We report that while the glycolytic pathway inT. saccharolyticumis as thermodynamically favorable as that found in model organisms, such as E. coli or Saccharomyces cerevisiae , the glycolytic pathway ofC. thermocellumoperates near equilibrium. The use of a near-equilibrium glycolytic pathway, with potentially increased ATP yield, by this cellulolytic microbe may represent an evolutionary adaptation to growth on cellulose, but it has the drawback of being highly susceptible to product feedback inhibition. The results of this study will facilitate future engineering of high-performance strains capable of transforming cellulosic biomass to biofuels at high yields and titers.