Transcriptome Analysis of Differentially Expressed Genes in Wild Jujube Seedlings under Salt Stress

作者：Xinyi Chang, Junli Sun, Lianling Liu, Wang He, Baolong Zhao · 发表于：Journal of the American Society for Horticultural Science · 年份：2020 · DOI：10.21273/jashs04801-19 · 被引用次数：24 · 研究领域：Ziziphus Jujuba Studies and Applications、Photosynthetic Processes and Mechanisms、Plant Molecular Biology Research

Wild jujube ( Ziziphus acidojujuba ) and cultivated jujube ( Ziziphus jujuba ) belong to the family Rhamnaceae. Jujubes have marked drought- and salt-tolerant properties. After salt stress, wild jujube seedling growth was inhibited and photosynthetic efficiency was reduced. A bioinformatics approach was used to analyze the transcriptomics data from wild jujube seedlings grown under salt stress, and the genes differentially expressed under the salt stress were identified to provide a theoretical basis for the development and use of wild jujube plantations in salinized soil. The transcriptome sequencing from leaves of wild jujube seedlings was carried out using second-generation sequencing technology. The effects of salt stress on the differential expression of photosynthesis-related genes in wild jujube seedlings were analyzed. Transcriptome sequencing revealed a total of 5269 differentially expressed genes (DEGs), of which 2729 were up-regulated and 2540 were down-regulated. DEGs were mainly enriched with respect to photosynthesis, photosynthetic antenna proteins, glyoxylic acid and dicarboxylic acid metabolism, linolenic acid metabolism, cysteine and methionine metabolism, and porphyrin and chlorophyll metabolism. Among them, the photosynthesis pathway-related DEGs were most highly enriched. Further analysis of porphyrin and chlorophyll synthesis and photosynthesis-related pathways revealed that they were significantly enriched by 97 photosynthesis-related DEGs. The DEGs in ...