Abundances of transcripts, proteins, and metabolites in the cell cycle of budding yeast reveal coordinate control of lipid metabolism

作者：Heidi M. Blank, Ophelia Papoulas, Nairita Maitra, Riddhiman K. Garge, Brian K. Kennedy, Birgit Schilling, Edward M. Marcotte, Michael Polymenis · 发表于：Molecular Biology of the Cell · 年份：2020 · DOI：10.1091/mbc.e19-12-0708 · 被引用次数：51 · 研究领域：Microbial Metabolic Engineering and Bioproduction、Lipid metabolism and biosynthesis、Advanced Proteomics Techniques and Applications

Establishing the pattern of abundance of molecules of interest during cell division has been a long-standing goal of cell cycle studies. Here, for the first time in any system, we present experiment-matched datasets of the levels of RNAs, proteins, metabolites, and lipids from unarrested, growing, and synchronously dividing yeast cells. Overall, transcript and protein levels were correlated, but specific processes that appeared to change at the RNA level (e.g., ribosome biogenesis) did not do so at the protein level, and vice versa. We also found no significant changes in codon usage or the ribosome content during the cell cycle. We describe an unexpected mitotic peak in the abundance of ergosterol and thiamine biosynthesis enzymes. Although the levels of several metabolites changed in the cell cycle, by far the most significant changes were in the lipid repertoire, with phospholipids and triglycerides peaking strongly late in the cell cycle. Our findings provide an integrated view of the abundance of biomolecules in the eukaryotic cell cycle and point to a coordinate mitotic control of lipid metabolism.