Aptamer-Conjugated Gold Nanoparticles Targeting Epidermal Growth Factor Receptor Variant III for the Treatment of Glioblastoma

作者：Li Peng, Yanling Liang, Xin-Xin Zhong, Zhiman Liang, Yinghong Tian, Shuji Li, Jingxue Liang, Ransheng Wang, Yuqi Zhong, Yusheng Shi, Xingmei Zhang · 发表于：International Journal of Nanomedicine · 年份：2020 · DOI：10.2147/ijn.s238206 · 被引用次数：88 · 研究领域：Advanced biosensing and bioanalysis techniques、Nuclear Structure and Function、Cell death mechanisms and regulation

PURPOSE: In this study, we constructed novel brain-targeting complexes (U2-AuNP) by conjugating aptamer U2 to the gold nanoparticle (AuNPs) surface as a promising option for GBM therapy. MATERIALS AND METHODS: The properties of the U2-AuNP complexes were thoroughly characterized. Then, we detected the in vitro effects of U2-AuNP in U87-EGFRvIII cell lines and the in vivo antitumor effects of U2-AuNP in GBM-bearing mice. Furthermore, we explored the inhibition mechanism of U2-AuNP in U87-EGFRvIII cell lines. RESULTS: We found that U2-AuNP inhibits the proliferation and invasion of U87-EGFRvIII cell lines and prolongs the survival time of GBM-bearing mice. We found that U2-AuNP can inhibit the EGFR-related pathway and prevent DNA damage repair in GBM cells. CONCLUSION: These results reveal the promising potential of U2-AuNP as a drug candidate for targeted therapy in GBM.