[The expression of p53, MDM2 and Ref1 gene in cultured retina neurons of SD rats treated with vitamin B1 and/or elevated pressure].

作者：Zhikuan Yang, Jian Ge, Wei Yin, Huangxuan Shen, Haiquan Liu, Yan Guo · 发表于：PubMed · 年份：2004 · 被引用次数：19 · 研究领域：Cancer-related Molecular Pathways、Drug-Induced Ocular Toxicity、Retinal and Macular Surgery

PURPOSE: To investigate the expression of p53, MDM2 and Ref1 gene in cultured retina neurons of SD rats treated with Vitamin B1 and (or) elevated pressure. METHODS: The retinal neuron of postnatal SD rats were cultured in vivo, the elevated pressure was produced after 7 days, and the total RNA was extracted after another 2 days, expression of p53, MDM2 and Ref1 gene were analyzed with RT-PCR. RESULTS: The expression level of p53 and MDM2 gene were increased in elevated pressure group, normal with Ref1 gene expression. But the expression of p53 and MDM2 gene were decreased significantly in elevated pressure group treated with vitamine B1 compare to the elevated group. CONCLUSION: Apoptosis seem to be a mechanism of cell death in retinal neurons of SD rats with elevated pressure.Vitamine B1 have protect effects against elevated pressure.