Rapid SPE – LC MS/MS analysis for atrazine, its by-products, simazine and S metolachlor in groundwater samples

作者：Hélène Blanchoud, Fabrice Alliot, Ningxin Chen, Danièle Valdés · 发表于：MethodsX · 年份：2020 · DOI：10.1016/j.mex.2020.100824 · 被引用次数：14 · 研究领域：Analytical chemistry methods development、Pesticide Residue Analysis and Safety、Pharmaceutical and Antibiotic Environmental Impacts

A rapid analysis of pesticides using on-line Solid phase extraction LC MS/MS (Agilent Technology) was performed using only 2-mL water samples. SPE cartridge PLRP-s was used for the pre-concentration sample with methanol elution in back flush. Sensitive transitions and mass spectrometry conditions were optimized by direct infusion of individual standard solutions in a positive electrospray mode. Water samples were spiked with internal standards to compensate the matrix effect. The limit of quantification was calculated to be 20 ng L −1 using the standard deviation of blank analysis injected ten times and uncertainties were estimated at less than 20% on concentrations. This method was validated to study leaching water samples for which only small quantities of water were available. •Only 2 mL water sample was used. •Samples were filtered at 0.2 µm and spiked with individual standard. •Compounds were separated in an 18.5-min elution time using the dynamic MRM program.