Biocompatible PEGDA Resin for 3D Printing

作者：Chandler A. Warr, Jonard Corpuz Valdoz, Bryce P. Bickham, Connor J. Knight, Nicholas A. Franks, Nicholas Chartrand, Pam M. Van Ry, Ken Christensen, Gregory P. Nordin, Alonzo D. Cook · 发表于：ACS Applied Bio Materials · 年份：2020 · DOI：10.1021/acsabm.0c00055 · 被引用次数：137 · 研究领域：3D Printing in Biomedical Research、Nanofabrication and Lithography Techniques、Additive Manufacturing and 3D Printing Technologies

We report a non-cytotoxic resin compatible with and designed for use in custom high-resolution 3D printers that follow the design approach described in Gong et al., Lab Chip 17, 2899 (2017). The non-cytotoxic resin is based on a poly(ethylene glycol) diacrylate (PEGDA) monomer with avobenzone as the UV absorber instead of 2-nitrophenyl phenyl sulfide (NPS). Both NPS-PEGDA and avobenzone-PEGDA (A-PEGDA) resins were evaluated for cytotoxicity and cell adhesion. We show that NPS-PEGDA can be made effectively non-cytotoxic with a post-print 12-hour ethanol wash, and that A-PEGDA, as-printed, is effectively non-cytotoxic. 3D prints made with either resin do not support strong cell adhesion in their as-printed state; however, cell adhesion increases dramatically with a short plasma treatment. Using A-PEGDA, we demonstrate spheroid formation in ultra-low adhesion 3D printed wells, and cell migration from spheroids on plasma-treated adherent surfaces. Given that A-PEGDA can be 3D printed with high resolution, it has significant promise for a wide variety of cell-based applications using 3D printed microfluidic structures.