[Expression of long noncoding RNA linc00261 in hepatocellular carcinoma and its association with postoperative outcomes].

作者：Zhanjun Chen, Leyang Xiang, Yu Huang, Yinghao Fang, Xianghong Li, Dinghua Yang · 发表于：PubMed · 年份：2018 · DOI：10.3969/j.issn.1673-4254.2018.10.05 · 被引用次数：10 · 研究领域：Cancer-related molecular mechanisms research、Cancer Mechanisms and Therapy、Medicinal Plant Pharmacodynamics Research

OBJECTIVE: To investigate the expression of long noncoding RNA linc00261 in hepatocellular carcinoma (HCC) and its correlation with the clinicopathological features and postoperative outcomes of the patients. METHODS: Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression of linc00261 in surgical specimens of HCC and adjacent tissues from 74 patients. The correlation of the expression level of linc00261 in HCC with the clinicopathological parameters of the patients was analyzed using Chi-square test. The Cox's proportional hazards regression model was used to assess the value of linc00261 in predicting the prognosis of HCC patients after operation. The expression of linc00261 was also examined in 5 HCC cell lines using qRT-PCR. The HCC cell lines MHCC-LM3 and SNU-449 were transfected with small interfering RNAs targeting linc00261 for linc00261 knockdown, and the changes in the cell proliferation, migration and invasion abilities were observed using CCK-8 assay and Transwell assay. RESULTS: > 0.05). CONCLUSIONS: Linc00261 may serve as a new prognostic biomarker for predicting the postoperative outcomes of the patients with HCC.