MIFlowCyt‐EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

作者：Joshua A Welsh, Edwin van der Pol, Ger J. A. Arkesteijn, Michel Bremer, Alain Brisson, Frank A. W. Coumans, Françoise Dignat‐George, E. Duggan, Ionita Ghiran, Bernd Giebel, André Görgens, An Hendrix, Romaric Lacroix, Joanne Lannigan, Sten F.W.M. Libregts, Estefanía Lozano−Andrés, Aizea Morales‐Kastresana, Stéphane Robert, Leonie de Rond, Tobias Tertel, John Tigges, Olivier De Wever, Xiaomei Yan, Rienk Nieuwland, Marca H. M. Wauben, John P. Nolan, Jennifer Jones · 发表于：Journal of Extracellular Vesicles · 年份：2020 · DOI：10.1080/20013078.2020.1713526 · 被引用次数：527 · 研究领域：Extracellular vesicles in disease、Lattice Boltzmann Simulation Studies

Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented many challenges and has produced a number of controversial results, in part due to limitations of instrument detection, lack of robust methods and ambiguities in how data should be interpreted. These complications are exacerbated by the field's lack of a robust reporting framework, and many EV-FC manuscripts include incomplete descriptions of methods and results, contain artefacts stemming from an insufficient instrument sensitivity and inappropriate experimental design and lack appropriate calibration and standardization. To address these issues, a working group (WG) of EV-FC researchers from ISEV, ISAC and ISTH, worked together as an EV-FC WG and developed a consensus framework for the minimum information that should be provided regarding EV-FC. This framework incorporates the existing Minimum Information for Studies of EVs (MISEV) guidelines and Minimum Information about a FC experiment (MIFlowCyt) standard in an EV-FC-specific reporting framework (MIFlowCyt-EV) that supports reporting of critical information related to sample staining, EV detection and measurement and experimental design in manuscripts that report EV-FC data. MIFlowCyt-EV provides a structure for sharing EV-FC results, but it does not prescribe specific prot...