Karrikins-induced accumulation of Salvianolic acid B is regulated by Jasmonic acid and hydrogen peroxide in Salvia miltiorrhiza

作者：Hui Sun, Zixin Xu, Jie Zhou, Lanping Guo · 发表于：South African Journal of Botany · 年份：2020 · DOI：10.1016/j.sajb.2020.01.021 · 被引用次数：11 · 研究领域：Plant Parasitism and Resistance、Plant-derived Lignans Synthesis and Bioactivity、Insect-Plant Interactions and Control

The influence of smoke-isolated karrikins (KAR 1 ) on the production of endogenous signal molecules and secondary metabolite Salvianolic acid B (SAB) in Salvia miltiorrhiza has been studied. These results indicated that KAR 1 induced increase of the accumulation of jasmonic acid (JA) and hydrogen peroxide (H 2 O 2 ) as well as SAB production. The contents of JA and H 2 O 2 were increased by treatment of KAR 1 , suggesting that production of JA and H 2 O 2 was early biochemical reaction of S. miltiorrhiza responding to KAR 1 in this experiment. KAR 1 -stimulated generation of JA and H 2 O 2 was inhibited by JA inhibitors, which indicated that KAR 1 -induced accumulation of JA was located in the upstream of H 2 O 2 biosynthesis. Treatments with JA-specific scavengers (salicylhydroxamic acid (SHAM) and n-propylgallate (PrGall)) and H 2 O 2 biosynthesis inhibitors (catalase (CAT) and diphenylene iodonium (DPI)) inhibited KAR 1 -induced accumulation of SAB, which suggested that JA and H 2 O 2 played signal roles in KAR 1 -stimulated accumulation of SAB. CAT/DPI could inhibit KAR 1 -induced promotion of SAB induced by exogenous JA. In addition, application of H 2 O 2 could reverse KAR 1 -induced inhibitory effects of SHAM, PrGall toward SAB production. Our results indicated that JA mediated the KAR 1 -induced increase of SAB dependent on the generation of H 2 O 2 in S. miltiorrhiza .