Evaluation of 1‐mm‐diameter endothelialized dense collagen tubes in vascular microsurgery

作者：Xuanyue Li, Jing Xu, Erzsébet Bartolák‐Suki, John Jiang, Joe Tien · 发表于：Journal of Biomedical Materials Research Part B Applied Biomaterials · 年份：2020 · DOI：10.1002/jbm.b.34576 · 被引用次数：16 · 研究领域：Electrospun Nanofibers in Biomedical Applications、Tissue Engineering and Regenerative Medicine、Bone Tissue Engineering Materials

Although much progress has been made in engineering vascular grafts for large- and small-diameter arterial repair or bypass, the extension of these results to the microsurgical size scale has been challenging. Here, we evaluated the use of dense collagen tubes (outer diameter 1 mm, inner diameter 0.5 mm) for vascular microsurgery as interpositional grafts to the femoral artery of Lewis rats. These tubes were formed by dehydrating tubular collagen gels around a mandrel, crosslinking them with genipin, seeding with syngeneic endothelial cells, and culturing before implantation by suture anastomosis. The retention of a confluent endothelial lining inside the tubes after mock surgical handling depended strongly on the crosslinker concentration and culture time. Optimized preparation conditions enabled retention of endothelium after mock surgical handling in ~80% of tubes and maintenance of patency 7 days after implantation in ~40% of grafts. Histological analysis showed the development of granulation tissue and the presence of CD31-positive structures on the inner and outer surfaces of implants. This study provides a proof-of-principle demonstration that endothelialized dense collagen tubes can remain patent for up to 7 days after vascular microsurgery, and points to the importance of mild scaffold crosslinking for maintaining firm endothelial adhesion.