Metabolomics of the Protective Effect of Ampelopsis grossedentata and Its Major Active Compound Dihydromyricetin on the Liver of High‐Fat Diet Hamster

作者：Lanlan Fan, Xiaosheng Qu, Tao Yi, Yong Peng, Manjing Jiang, Jianhua Miao, Peigen Xiao · 发表于：Evidence-based Complementary and Alternative Medicine · 年份：2020 · DOI：10.1155/2020/3472578 · 被引用次数：28 · 研究领域：Medicinal plant effects and applications、Biological Activity of Diterpenoids and Biflavonoids、Natural product bioactivities and synthesis

The flavonoid dihydromyricetin (DMY) is the main component of Ampelopsis grossedentata (Hand‐Mazz) W. T. Wang (AG), a daily beverage and folk medicine used in Southern China to treat jaundice hepatitis, cold fever, and sore throat. Recently, DMY and AG were shown to have a beneficial effect on lipid metabolism disorder. However, the mechanisms of how DMY and AG protect the liver during lipid metabolism disorder remain unclear. In this study, we first analyzed the chemical compounds of AG by HPLC‐DAD‐ESI‐IT‐TOF‐MS n . Of the 31 compounds detected, 29 were identified based on previous results. Then, the effects of DMY and AG on high‐fat diet hamster livers were studied and the metabolite levels and metabolic pathway activity of the liver were explored by 1 H NMR metabolomics. Compared to the high‐fat diet group, supplementation of AG and DMY attenuated the high‐fat‐induced increase in body weight, liver lipid deposition, serum triglycerides and total cholesterol levels, and normalized endogenous metabolite concentrations. PCA and PLS‐DA score plots demonstrated that while the metabolic profiles of hamsters fed a high‐fat diet supplemented with DMY or AG were both far from those of hamsters fed a normal diet or a high‐fat diet alone, they were similar to each other. Our data suggest that the underlying mechanism of the protective effect of DMY and AG might be related to an attenuation of the deleterious effect of high‐fat diet‐induced hyperlipidemia on multiple metabolic pathway...