[Effect of stromal cell-derived factor 1α/cysteine X cysteine receptor 4 signaling pathway on axial stress stimulation promoting bone regeneration].

作者：Jianbiao Lin, Guofeng Huang, Wenbin Ye, Cong Zhu, Jianting Gao, Guojun Liu, Huixiang Jiang, Benwen Wu, Zhenqi Ding · 发表于：PubMed · 年份：2019 · DOI：10.7507/1002-1892.201811031 · 被引用次数：2 · 研究领域：Connective Tissue Growth Factor Research、Bone Metabolism and Diseases、Periodontal Regeneration and Treatments

OBJECTIVE: To observe the change of stromal cell-derived factor 1α/cysteine X cysteine receptor 4 (SDF-1α/CXCR4) signaling pathway during the process of axial stress stimulation promoting bone regeneration, and to further explore its mechanism. METHODS: =24). Group A was treated with intraperitoneally injection of PBS; Group B was treated with stress stimulation and intraperitoneally injection of PBS; Group C was treated with stress stimulation and intraperitoneally injection of AMD3100 solution. The X-ray films were taken and Lane-Sandhu scores of bone healing were scored at 2, 4, 8, and 12 weeks after operation, while specimens were harvested for HE staining, immunohistochemical staining of vascular endothelial growth factor (VEGF) and CXCR4, and Western blot (SDF-1α and CXCR4). The bone healing area was scanned by Micro-CT at 12 weeks after operation, and the volume and density of new bone were calculated. RESULTS: <0.05). CONCLUSION: Axial stress stimulation can promote the expression of SDF-1α in bone defect tissue, activate and regulate the CXCR4 signal collected by marrow mesenchymal stem cells, and accelerate bone regeneration in bone defect area.