Genome‐Wide Profiling of Human Papillomavirus DNA Integration into Human Genome and Its Influence on PD‐L1 Expression in Chinese Uygur Cervical Cancer Women

作者：Yangchun Feng, Wang Sen-yu, Yuan Zhang, Yanchun Huang · 发表于：Journal of Immunology Research · 年份：2020 · DOI：10.1155/2020/6284960 · 被引用次数：26 · 研究领域：Cervical Cancer and HPV Research、Cancer-related molecular mechanisms research、Genetic factors in colorectal cancer

Background . The Uygur is the fifth most populous ethnic group in China. Compared to other Chinese population, cervical cancer in them had high incidence, and HPV infection also was particular. Their HPV integration situation has never been reported. We aimed to investigate the integration situation of 20 subtypes of HPV gene into host cell genome in Chinese Uygur cervical cancer patients; meanwhile, we explored the influence of gene integration on PD‐L1 expression. Methods . 40 frozen Chinese Uygur cervical cancer specimens with positive HPV infection were obtained from the cancer prevention and treatment institute of Tumor Hospital Affiliated to Xinjiang Medical University. The integration situation of HPV gene into host cell genome was detected by Agilent SureSelect™ Target Enrichment Chip and Next‐Generation Sequencing. The related genes were analyzed by GO functional annotation and KEGG pathway enrichment. The expression levels of PD‐L1 in cancer cells were tested by immunohistochemical assay (IHC). Meanwhile, the relationship between PD‐L1 levels in cancer cells and gene integration were analyzed. Results . The HPV multiple infection rate by HIVID was as high as 92.5%, much higher than 35.0% by the commercial kit ( P < 0.05). There were 13423 integration events in 40 specimens, involving 6867 human genes. These integration events were distributed on all human chromosomes, and chromosome 19 had the excessive concentration phenomenon of integration events. There were s...