Toxicity, Biodegradation, and Metabolic Fate of Organophosphorus Pesticide Trichlorfon on the Freshwater Algae Chlamydomonas reinhardtii

作者：Liang Wan, Yixiao Wu, Huijun Ding, Weihao Zhang · 发表于：Journal of Agricultural and Food Chemistry · 年份：2020 · DOI：10.1021/acs.jafc.9b05765 · 被引用次数：115 · 研究领域：Environmental Toxicology and Ecotoxicology、Pesticide and Herbicide Environmental Studies、Pharmaceutical and Antibiotic Environmental Impacts

This study investigated the toxicity of trichlorfon (TCF) to the freshwater algae Chlamydomonas reinhardtii, as well as its biodegradation and metabolic fate. The growth of C. reinhardtii decreased with increasing TCF concentration, and the maximum inhibition ratio was 51.3% at 200 mg L –1 TCF compared to the control. Analyses of pigment content, chlorophyll fluorescence, and antioxidant enzymes indicated that C. reinhardtii can produce resistance and acclimatize to the presence of TCF. The variations in pH during cultivation suggested that photosynthetic microalgae have innate advantages over bacteria and fungi in remediating TCF. A 100% biodegradation rate was achieved at a maximum concentration of 100 mg L –1 TCF. Ten metabolites were identified by GC–MS, and the degradation pathways of TCF by the algae were proposed. This research demonstrated that C. reinhardtii is highly tolerant to and can efficiently degrade TCF. Thus, C. reinhardtii can be used to remove traces of TCF from natural water environments and to treat TCF-contaminated wastewater.