Effects of 14-year continuous nitrogen addition on soil arylsulfatase and phosphodiesterase activities in a mature tropical forest

作者：Taiki Mori, Kaijun Zhou, Cong Wang, Senhao Wang, Ying‐Ping Wang, Mianhai Zheng, X. L. Lu, Wei Zhang, Jiangming Mo · 发表于：Global Ecology and Conservation · 年份：2020 · DOI：10.1016/j.gecco.2020.e00934 · 被引用次数：16 · 研究领域：Soil Carbon and Nitrogen Dynamics、Legume Nitrogen Fixing Symbiosis、Cassava research and cyanide

We investigated the impacts of 14-year continuous N addition on activities of arylsulfatase (AS) and phosphodiesterase (PDE), which catalyze soil organic sulfur (S) and phosphorus (P), respectively. The response of AS to N addition was compared with that of C- and N-acquiring enzymes, i.e., β-1,4-glucosidase (BG), β-D-cellobiohydrolase (CBH), β-1,4-Xylosidase (BX), β-1,4-N-acetyl-glucosaminnidase (NAG), and leucine amino peptidase (LAP). We also compared the impact of N addition on PDE activity with that on phosphomonoesterase (PME) activity. The results showed that N addition clearly decreased soil AS activity, whereas activities of C- and N-acquiring enzymes did not exhibit similar changes. The inconsistent response of AS activity with the enzymes was attributed to soil acidification induced by long-term N addition, which shifts the pH condition to a more optimal condition for AS activity and accelerates the accumulation of S in soils. The ratio of PME to PDE were significantly elevated by N addition, implying that the microbial and plant resource allocation to PME relative to PDE increased after N addition at our study site. The shift in resource allocation may have occurred because (i) the types of phosphatases secreted by biota shifted toward phosphatases that require one-step reaction to obtain P (such as PME) in the more-severely P-depleted condition, or (ii) soil acidification provided a more optimal condition for PDE than PME, which resulted in lower PDE production a...