Quantitative Trait Locus Analysis of Seed Germination and Early Seedling Growth in Rice

作者：Jing Yang, Guili Yang, Meng Yang, Ling Su, Aoyun Xia, Dandan Li, Cuihong Huang, Danhua Zhou, Yongzhu Liu, Hui Wang, Zhiqiang Chen, Tao Guo · 发表于：Frontiers in Plant Science · 年份：2019 · DOI：10.3389/fpls.2019.01582 · 被引用次数：30 · 研究领域：Genetic Mapping and Diversity in Plants and Animals、Rice Cultivation and Yield Improvement、GABA and Rice Research

the genotyping-by-sequencing (GBS) approach to construct a high-density linkage bin map based on the parent-independent genotyping method. Quantitative trait loci (QTLs) for 12 traits related to seed germination and early seedling growth were analyzed. Totally, 22 additive loci were detected, after analysis of the interaction between additive QTLs and environments, five stable additive loci were obtained. Among them, loci 4, 5, 12 and 14 exhibited clear pleiotropic effects that were associated with multiple traits. Analysis of the effects of the five additive stable loci showed that a single locus increased the corresponding phenotypic value. Ten of the 275 RILs pyramided the excellent alleles of the five stable genetic loci. Most phenotypic values of the ten RILs were greater than the average values. Four RILs (G260, G342, G371, and G401) with more excellent phenotypic values were subsequently selected; these RILs could serve as donor parents of favorable alleles in the breeding process. Due to the existence of pleiotropy, the use of these genetic loci for pyramid breeding can further increase the efficiency to reach breeding goals. In addition, these five stable loci have an average physical interval of only 170 kb, we also further identified five promising candidate genes by qRT-PCR, which provides us with a basis for future cloning of these genes. Overall, this work will help broaden our understanding of the genetic control of seed germination and early seedling growth, a...