Real-time imaging of infarction deterioration after ischemic stroke in rats using electrical impedance tomography

作者：Lu Cao, Haoting Li, Danchen Fu, Xuechao Liu, Hang Ma, Canhua Xu, Xiuzhen Dong, Bin Yang, Feng Fu · 发表于：Physiological Measurement · 年份：2020 · DOI：10.1088/1361-6579/ab69ba · 被引用次数：25 · 研究领域：Electrical and Bioimpedance Tomography、Microfluidic and Bio-sensing Technologies、Body Composition Measurement Techniques

OBJECTIVE: This study investigated the feasibility of electrical impedance tomography (EIT) for monitoring the deterioration of ischemic lesion after the onset of stroke. APPROACH: Fifteen rats were randomly distributed into two groups: rats operated to establish a right middle cerebral artery occlusion (MCAO) (n = 10), and sham-operated rats (n = 5). Then, the operated rats were kept 2 h under anesthesia for EIT monitoring. Subsequently, descriptive statistical analysis was performed on whole-brain resistivity changes, and repeated-measures analysis of variance (ANOVA) on the average resistivity variation index. Additionally, pathological examinations were performed after 6 h of infarction. MAIN RESULTS: The results obtained showed that ischemic damage developed in the right corpus striatum of the rats with MCAO, whereas the brains of the sham group showed no anomalies. The descriptive statistical analysis revealed that the whole-brain resistivity changes after 30, 60, 90, and 120 min of infarction were 0.063 ± 0.038, 0.097 ± 0.046, 0.141 ± 0.062, and 0.204 ± 0.092 for the rats with MCAO and 0.029 ± 0.021, 0.002 ± 0.002, 0.017 ± 0.011, and -0.001 ± 0.011 for the sham-operated rats, respectively. The repeated-measures ANOVA revealed that the right MCAO model resulted in a significant impedance increase in the right hemisphere, which continued to increase over time after infarction. SIGNIFICANCE: The overall study results indicate that EIT facilitates monitoring of local imped...