Chain conformation transition induced host–guest assembly between triple helical curdlan and β -CD for drug delivery

作者：Qingye Liu, Yang Du, Tongyi Shang, Lixiao Guo, Bin Yang, Xiaojuan Xu · 发表于：Biomaterials Science · 年份：2020 · DOI：10.1039/c9bm01439j · 被引用次数：16 · 研究领域：Supramolecular Self-Assembly in Materials、Carbohydrate Chemistry and Synthesis、Mesoporous Materials and Catalysis

The unique conformation transition from a triple helix to single coils for the triple helical β-d-glucans has paved the way to fabricate various functional nanocomposites through the denaturing-renaturing process. This study firstly reports a novel kind of naturally derived supramolecular polymer micelle consisting of single-stranded chains of curdlan (CUR) and β-CDs. It is proposed that β-CDs as the host molecules were threaded onto single β-glucan chains (denatured triplex CUR) via the host-guest interaction, thereby forming supramolecular micelles. The results from the 1H NMR, FT-IR, XRD and 2D 1H NOESY NMR studies confirmed the formation of the inclusion complex and the existence of the core-shell structure of the supramolecular assembly. TEM images and DLS revealed that the self-organized micelles displayed a regular spherical shape with an average diameter of ∼27 nm. Furthermore, the hydrophobic anticancer drug camptothecin (CPT) was selected as a model drug and successfully encapsulated into the CUR/β-CD micelles. The drug-loaded micelles exhibited a steady sustained-release pattern regardless of the environmental pH. The flow cytometry and confocal laser scanning microscopy measurements confirmed that the CPT-loaded micelles could be well internalized into HepG 2 cells and continuously release the drug molecules inside the tumor cells. Meanwhile, the in vivo experiments demonstrated that CPT-loaded micelles could effectively inhibit tumor growth in comparison to free ...