Corona of Thorns: The Surface Chemistry-Mediated Protein Corona Perturbs the Recognition and Immune Response of Macrophages

作者：Rong Cai, Jiayu Ren, Yinglu Ji, Yaling Wang, Ying Liu, Zhiqiang Chen, Zeinab Farhadi Sabet, Xiaochun Wu, Iseult Lynch, Chunying Chen · 发表于：ACS Applied Materials & Interfaces · 年份：2019 · DOI：10.1021/acsami.9b15910 · 被引用次数：151 · 研究领域：Nanoparticle-Based Drug Delivery、RNA Interference and Gene Delivery、Phagocytosis and Immune Regulation

The significance of protein coronas on the biological fates of nanoparticles has been widely recognized. Therefore, the alterations on biological effects caused by protein coronas need systemic study and interpretation to design novel safe and efficient nanomedicines. In the present study, we present a comprehensive quantitative analysis of the protein coronas on gold nanorods modified with various surface ligands of different chemical compositions and charges. The design of surface ligands is of utmost importance for the functionalization of nanoparticles, and further, the ligand-induced biological identity determines the fate of nanoparticles in the human body. We found that the surface chemistry influences the composition of the protein corona more profoundly than surface charge. Since the first and most important challenge for administrated nanomedicines is navigating the interaction with macrophages, we further investigated how the surface chemistry-induced specific protein corona affects the phagocytosis and immune responses of macrophages exposed to the corona-nanoparticle complexes. Our results reveal that the protein corona alters the internalization pathways of gold nanorods by macrophages via the interactions of the predominant coronal proteins with specific receptors on the cell membrane. The cytokine secretion profile of macrophages is also highly dependent on the adsorption pattern of the protein corona. The more abundant proteins involved in immune responses, s...