Genetic variation of Echinococcus spp. in yaks and sheep in the Tibet Autonomous Region of China based on mitochondrial DNA

作者：John Asekhaen Ohiolei, Chenyang Xia, Li Li, Jianzhi Liu, Wenqiang Tang, Yan‐Tao Wu, Danqulamu, Guoqiang Zhu, Bin Shi, Bao‐Quan Fu, Hong Yin, Hongbin Yan, Wan‐Zhong Jia · 发表于：Parasites & Vectors · 年份：2019 · DOI：10.1186/s13071-019-3857-1 · 被引用次数：31 · 研究领域：Parasitic infections in humans and animals、Amoebic Infections and Treatments、Echinoderm biology and ecology

Abstract Background Cystic echinococcosis (CE) in humans and livestock is caused by Echinococcus granulosus ( sensu lato ). In China where CE is endemic, a number of studies have shown that Echinococcus granulosus ( sensu stricto ) is majorly responsible for CE. However, E. canadensis (G6) which is the second leading cause of CE is now being detected in most parts of the country. In this study, the species diversity and genetic variation of Echinococcus granulosus ( s.l. ) in four counties in Tibet Autonomous Region of China were investigated. Methods Infection with Echinococcus granulosus ( s.s. ) in yaks and sheep was identified using NADH dehydrogenase subunit 1 and 5 ( nad 1 and nad 5) mitochondrial genes while the genotype G6 of E. canadensis initially diagnosed with NADH dehydrogenase subunit 1 ( nad 1) was further confirmed by analysis of the complete mitochondrial genome and a phylogenetic network constructed based on the nad 2 and nad 5 genes. Results Out of 85 hydatid cyst samples collected from slaughtered sheep ( n = 54) and yaks ( n = 31), 83 were identified as E. granulosus ( s.s. ) G1 ( n = 77), G3 ( n = 6) and 2 were identified as E. canadensis G6. Analysis of the nad 1/ nad 5 genes revealed 16/17 mutations with 9/14 parsimony informative sites resulting in 15/14 haplotypes, respectively. Haplotype diversity (Hd) and nucleotide diversity (π) of E. granulosus ( s.s. ) population were 0.650 and 0.00127 for nad 1 and 0.782 and 0.00306 for nad 5, respectively, wit...