Stiffness of cationized gelatin nanoparticles is a key factor determining RNAi efficiency in myeloid leukemia cells

作者：Weiqi Zhang, Bo Hoon Han, Xinning Lai, Chen Xiao, Shilin Xu, Xianghui Meng, Zifu Li, Jie Meng, Tao Wen, Xiangliang Yang, Jian Liu, Haiyan Xu · 发表于：Chemical Communications · 年份：2019 · DOI：10.1039/c9cc09068a · 被引用次数：30 · 研究领域：RNA Interference and Gene Delivery、Advanced biosensing and bioanalysis techniques、DNA and Nucleic Acid Chemistry

Here we demonstrated that the stiffness of cationized gelatin nanoparticles determined the efficiency of RNAi in myeloid leukemia cells when the particle size and surface charges were kept constant. The siRNA delivery system with an elastic modulus of 0.87 MPa showed the largest siRNA uptake and RNAi efficiency for hard-to-transfect suspension cells.