In vitro expansion of human sperm through nuclear transfer

作者：Xiaoyu Merlin Zhang, Keliang Wu, Yuxuan Zheng, Han Zhao, Junpeng Gao, Zhenzhen Hou, Meiling Zhang, Jiaoyang Liao, Jing‐ye Zhang, Yuan Gao, Yuanyuan Li, Li Lin, Fuchou Tang, Zi‐Jiang Chen, Jinsong Li · 发表于：Cell Research · 年份：2019 · DOI：10.1038/s41422-019-0265-1 · 被引用次数：24 · 研究领域：Pluripotent Stem Cells Research、Animal Genetics and Reproduction、CRISPR and Genetic Engineering

Mammalian haploid embryonic stem cells (haESCs) represent an ideal tool for genetic analysis due to the presence of only one set of genetic materials. HaESCs fall into two readily distinguishable groups based on their genome origins: parthenogenetic haESCs (PG-haESCs) that are derived from oocyte-originated parthenogenetic embryos and androgenetic haESCs (AG-haESCs) that are produced through sperm nuclear transfer. Both PG- and AG-haESCs are feasible for delineating genome function at cellular level in vitro. Importantly, AG-haESCs can be used as a sperm replacement and applied to deciphering gene function at organismal level when combined with CRISPR-Cas9 technology. 1 However, while PG-haESCs have been generated from both rodent and primates, 2 , 3 , 4 , 5 AG-haESCs can only be obtained in rodent to date. 6 , 7