[Diagnostic value of P2X7 receptor and its role in inflammatory reaction in rheumatoid arthritis].

作者：Yonghe Chen, Baochang Su, Mengqiao Shang · 发表于：PubMed · 年份：2018 · DOI：10.12122/j.issn.1673-4254.2018.12.09 · 被引用次数：4 · 研究领域：Adenosine and Purinergic Signaling、Spondyloarthritis Studies and Treatments、Rheumatoid Arthritis Research and Therapies

OBJECTIVE: To study the diagnostic value of P2X7 receptor for rheumatoid arthritis (RA) and its role in the inflammatory response. METHODS: With the synovial tissues from 25 patients with bone and joint replacement as the control,the synovial tissues of 25 RA patients were examined for the relative expression of P2X7 receptor mRNA using qRT-PCR.In an immortalized RA synovial cell line (MH7A),the effect of P2X7 receptor knockdown via a small interfering RNA were examined on the productions of the inflammatory cytokines including interleukin-1β(IL-1β),IL-6,and IL-8 using ELISA. RESULTS: <0.001).In MH7A cells,P2X7 receptor knockdown obviously reduced the secretion of IL-1β and IL-6. CONCLUSIONS: RA patients show elevated P2X7 receptor level in the synovial tissue, which has a good diagnostic value for RA.Blocking P2X7 receptor can inhibit inflammatory factor secretion and suppress inflammatory reactions.