Recent hybridization and allopolyploidy reprogrammed Spartina microRNA expression under xenobiotic induced stress

作者：Armand Cavé‐Radet, Armel Salmon, Loup Tran Van Canh, Richard Moyle, Lara-Simone Pretorius, Oscar Lima, Malika L. Aïnouche, Abdelhak El Amrani · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2019 · DOI：10.1101/2019.12.13.875138 · 被引用次数：1 · 研究领域：Plant Molecular Biology Research、Plant tissue culture and regeneration、Plant Stress Responses and Tolerance

Abstract Xenobiotic detoxification is a common trait of all living organisms, necessary for developmental plasticity and stress tolerance. The gene set involved in this biological process is dubbed the xenome ( i.e. involved in drug metabolism in mammals, degradation of allelochemicals and environmental pollutants by bacteria and plant communities). Recently, we found that allopolyploidy increased tolerance to xenobiotics (phenanthrene) in Spartina . To decipher the molecular mechanisms underlying this process, we examined how interspecific hybridization and genome doubling impact miRNAs expression under xenobiotic induced stress. In this work we used a deep sequencing approach, and analyzed the parental species S. alterniflora and S. maritima , their F1 hybrid S. x townsendii and the allopolyploid S. anglica under phenanthrene exposure. We found that hybridization and genome doubling reprogrammed a myriad of miRNAs under phenanthrene-induced stress. Hence, to identify the master miRNAs involved in phenanthrene tolerance, we performed experimental functional validation of phenanthrene-responsive Spar-miRNAs using Arabidopsis T-DNA mutant lines inserted in homologous MIR genes, 39 knock out T-DNA Arabidopsis mutants, tagged in the most conserved miRNAs genes in vascular plants were screened. Development of MIR159 and MIR156 mutants was significantly affected under phenanthrene-induced stress. Subsequently, we performed in planta experimental validation to confirm the interacti...