Evolution of resistance to HPPD‐inhibiting herbicides in a wild radish population via enhanced herbicide metabolism

作者：Huan Lu, Qin Yu, Heping Han, Mechelle Owen, Stephen B. Powles · 发表于：Pest Management Science · 年份：2019 · DOI：10.1002/ps.5725 · 被引用次数：73 · 研究领域：Weed Control and Herbicide Applications、Pesticide and Herbicide Environmental Studies、Allelopathy and phytotoxic interactions

Abstract BACKGROUND Relatively new herbicides that target 4‐hydroxyphenylpyruvate dioxygenase (HPPD) are now available for use on the world's great grain crops (rice, wheat, corn and soybean) and for other uses. With widespread and persistent use of HPPD‐inhibiting herbicides, the evolution of HPPD‐inhibiting herbicide resistant weeds is inevitable. Currently, resistance to HPPD‐inhibiting herbicides is known in two weed species, waterhemp and Palmer amaranth. Here, we report a HPPD‐inhibiting herbicide resistant wild radish population from the Western Australia grain belt. This population was not selected with HPPD‐inhibiting herbicides, rather it evolved resistance to earlier used herbicides with different modes of action and exhibits cross‐resistance to HPPD‐inhibiting herbicides. RESULTS Dose–response experiments showed the resistant (R) population exhibits 4 to 6.5‐fold resistance to the HPPD‐inhibiting herbicides mesotrione, tembotrione and isoxaflutole, compared to the susceptible (S) population. This resistance is not target‐site based as cloning of full coding sequences of the HPPD genes from S and R plants did not reveal resistance‐endowing single nucleotide polymorphisms. The HPPD gene expression levels are similar in S and R plants. In addition, no differences in [ 14 C]‐mesotrione uptake and translocation were observed in the S and R plants. However, the time required for R plants to metabolise 50% [ 14 C]‐mesotrione is 7.7‐fold faster than for the S plants. CONC...