Monocytes undergo multi-step differentiation in mice during oral infection by Toxoplasma gondii

作者：Aurélie Detavernier, Abdulkader Azouz, Hussein Shehade, Marion Splittgerber, Laurye Van Maele, Muriel Nguyen, Séverine Thomas, Younès Achouri, Dávid Švec, Emilie Calonne, François Fuks, Guillaume Oldenhove, Stanislas Goriely · 发表于：Communications Biology · 年份：2019 · DOI：10.1038/s42003-019-0718-6 · 被引用次数：18 · 研究领域：Toxoplasma gondii Research Studies、Cytomegalovirus and herpesvirus research、interferon and immune responses

Abstract Monocytes play a major role in the defense against pathogens. They are rapidly mobilized to inflamed sites where they exert both proinflammatory and regulatory effector functions. It is still poorly understood how this dynamic and exceptionally plastic system is controlled at the molecular level. Herein, we evaluated the differentiation process that occurs in Ly6C hi monocytes during oral infection by Toxoplasma gondii . Flow cytometry and single-cell analysis revealed distinct activation status and gene expression profiles in the bone marrow, the spleen and the lamina propria of infected mice. We provide further evidence that acquisition of effector functions, such as the capacity to produce interleukin-27, is accompanied by distinct waves of epigenetic programming, highlighting a role for STAT1/IRF1 in the bone marrow and AP-1/NF-κB in the periphery. This work broadens our understanding of the molecular events that occur in vivo during monocyte differentiation in response to inflammatory cues.