The human secretome

作者：Mathias Uhlén, Max Karlsson, Andreas Hober, Anne-Sophie Svensson, Julia Scheffel, David Kotol, Wen Zhong, Abdellah Tebani, Linnéa Strandberg, Fredrik Edfors, Evelina Sjöstedt, Jan Mulder, Adil Mardinoğlu, Anna Berling, Siri Ekblad, Melanie Dannemeyer, Sara Kanje, Johan Rockberg, Magnus Lundqvist, Magdalena Malm, Anna-Luisa Volk, Peter Nilsson, Anna Månberg, Tea Dodig‐Crnković, Elisa Pin, Martin Zwahlen, Per Oksvold, Kalle von Feilitzen, Ragna S. Häussler, Mun‐Gwan Hong, Cecilia Lindskog, Fredrik Pontén, Borbala Katona, Jimmy Vuu, Emil Lindström, Jens Nielsen, Jonathan L. Robinson, Burcu Ayoglu, Diana Mahdessian, Devin P. Sullivan, Peter Thul, Frida Danielsson, Charlotte Stadler, Emma Lundberg, Göran Bergström, Anders Gummesson, Björn G. Voldborg, Hanna Tegel, Sophia Hober, Björn Forsström, Jochen M. Schwenk, Linn Fagerberg, Åsa Sivertsson · 发表于：Science Signaling · 年份：2019 · DOI：10.1126/scisignal.aaz0274 · 被引用次数：534 · 研究领域：Machine Learning in Bioinformatics、Advanced Proteomics Techniques and Applications、Bioinformatics and Genomic Networks

The proteins secreted by human cells (collectively referred to as the secretome) are important not only for the basic understanding of human biology but also for the identification of potential targets for future diagnostics and therapies. Here, we present a comprehensive analysis of proteins predicted to be secreted in human cells, which provides information about their final localization in the human body, including the proteins actively secreted to peripheral blood. The analysis suggests that a large number of the proteins of the secretome are not secreted out of the cell, but instead are retained intracellularly, whereas another large group of proteins were identified that are predicted to be retained locally at the tissue of expression and not secreted into the blood. Proteins detected in the human blood by mass spectrometry-based proteomics and antibody-based immunoassays are also presented with estimates of their concentrations in the blood. The results are presented in an updated version 19 of the Human Protein Atlas in which each gene encoding a secretome protein is annotated to provide an open-access knowledge resource of the human secretome, including body-wide expression data, spatial localization data down to the single-cell and subcellular levels, and data about the presence of proteins that are detectable in the blood.