Identification and Expression Pattern Analysis of Bacterial Blight Resistance Genes in Oryza officinalis Wall ex Watt Under Xanthomonas oryzae Pv. oryzae Stress

作者：Chunmiao Jiang, Suqin Xiao, Dingqin Li, Ling Chen, Qiaofang Zhong, Fuyou Yin, Tengqiong Yu, Xue Ke, Dunyu Zhang, Jian Fu, Yue Chen, Bo Wang, Lingxian Wang, Exian Li, Yun Zhang, Xingqi Huang, Zaiquan Cheng · 发表于：Plant Molecular Biology Reporter · 年份：2019 · DOI：10.1007/s11105-019-01164-3 · 被引用次数：10 · 研究领域：Plant Pathogenic Bacteria Studies、Plant-Microbe Interactions and Immunity、Plant pathogens and resistance mechanisms

Abstract Background Bacterial blight (BB) caused by Xanthomonas oryzae Pv. oryzae ( Xoo ) is one of the most serious diseases of rice worldwide. Oryza officinalis Wall ex Watt, harboring abundant genetic diversity and disease resistance features, are important resources of exploring resistance genes with broad-spectrum resistance to BB. However, the molecular mechanisms and genes of BB resistance in O. officinalis have been rarely explored. Results Here, the BB resistance of four different origin O. officinalis populations in Yunnan were identified by seven representative hypervirulent Xoo races, which exhibited different BB resistance among four populations, in which the BB resistance of the Gengma_Lincang population was the strongest. In addition, the pathogenetic ability of seven Xoo races to O. officinalis was different in that the pathogenicity of PXO99 was stronger than that of C5. There were no remarkable differences in leaf microstructures among four O. officinalis populations, revealing the differences in resistance of four O. officinalis to BB are caused by the endogenous resistance genes. Furthermore, our results proved that there were no nine cloned BB resistance genes in four populations but possessed dominant Xa5 , dominant Xa13 , and recessive xa3/xa26 homologous alleles of xa5 , xa13 , and Xa3 / Xa26 resistance genes. These three homologous genes were isolated and cloned from four populations and named OoXa5 , OoXa13 , and Ooxa3 / xa26 . The expression profile...