Comparative Investigation into Formycin A and Pyrazofurin A Biosynthesis Reveals Branch Pathways for the Construction of C -Nucleoside Scaffolds

作者：Meng Zhang, Peichao Zhang, Gudan Xu, Wenting Zhou, Yaojie Gao, Rong Gong, You‐Sheng Cai, Hengjiang Cong, Zixin Deng, Neil P. J. Price, Xiangzhao Mao, Wenqing Chen · 发表于：Applied and Environmental Microbiology · 年份：2019 · DOI：10.1128/aem.01971-19 · 被引用次数：31 · 研究领域：Carbohydrate Chemistry and Synthesis、Biochemical and Molecular Research、Microbial Natural Products and Biosynthesis

FOR-A and PRF-A are C -nucleoside antibiotics known for their unusual chemical structures and remarkable biological activities. Deciphering the enzymatic mechanism for the construction of a C -nucleoside scaffold during FOR-A/PRF-A biosynthesis will not only expand the biochemical repertoire for novel enzymatic reactions but also permit target-oriented genome mining of FOR-A/PRF-A-related C -nucleoside antibiotics. Moreover, the availability of FOR-A/PRF-A biosynthetic gene clusters will pave the way for the rational generation of designer FOR-A/PRF-A derivatives with enhanced/selective bioactivity via synthetic biology strategies.