A portable and cost-effective microfluidic system for massively parallel single-cell transcriptome profiling

作者：Chuanyu Liu, Tao Wu, Fan Fei, Ya Liu, Liang Wu, Michael Junkin, Zhifeng Wang, Yeya Yu, Weimao Wang, Wenbo Wei, Yue Yuan, Mingyue Wang, Mengnan Cheng, Xiaoyu Wei, Jiangshan Xu, Quan Shi, Shiping Liu, Ao Chen, Ou Wang, Ming Ni, Wenwei Zhang, Zhouchun Shang, Yiwei Lai, Pengcheng Guo, Carl Ward, Giacomo Volpe, Lei Wang, Huan Zheng, Yang Liu, Brock A. Peters, Jody E. Beecher, Yong‐Wei Zhang, Miguel A. Esteban, Yong Hou, Xun Xu, I-Jane Chen, Longqi Liu · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2019 · DOI：10.1101/818450 · 被引用次数：96 · 研究领域：Single-cell and spatial transcriptomics、Cancer Genomics and Diagnostics、Microfluidic and Bio-sensing Technologies

Abstract Single-cell technologies are becoming increasingly widespread and have been revolutionizing our understanding of cell identity, state, diversity and function. However, current platforms can be slow to apply to large-scale studies and resource-limited clinical arenas due to a variety of reasons including cost, infrastructure, sample quality and requirements. Here we report DNBelab C4 (C4), a negative pressure orchestrated, portable and cost-effective device that enables high-throughput single-cell transcriptional profiling. C4 system can efficiently allow discrimination of species-specific cells at high resolution and dissect tissue heterogeneity in different organs, such as murine lung and cerebral cortex. Finally, we show that the C4 system is comparable to existing platforms but has huge benefits in cost and portability and, as such, it will be of great interest for the wider scientific community.